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KMID : 0545120020120030518
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 3 p.518 ~ p.521
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Cometabolic Production of Poly(3-Hydroxyalkanoates) Containing Carbon-Carbon Double and Triple Bonds by Pseudomonas olevorans
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KIM, DO YOUNG
KIM, YOUNG BAEK/RHEE, YOUNG HA
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Abstract
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Poly(3-hydroxyalkanoate) copolyesters containing both carbon-carbon double and carbon-carbon triple bonds were produced by Pseudomonas oleovorans grown in mixtures of 10-undecynoic acid (10-UND(≡)) and 10-undecenoic acid (10-UND(=)). The PHA content in the dry cells was usually 40 wt%. The bioconversion yield of 10-UND(≡) to PHA by P. oleovorans was remarkably enhanced from 1% to over 24% as the fraction of 10-UND(=) in the carbon substrate mixtures increased from 0 to 50%. These values were higher than those obtained when P. oleovorans was grown in the same molar mixtures of 10-UND(≡) and nonanoic acid (NA), indicating that 10-UND(=) was more efficient than NA as a cosubstrate in inducing cometabolic PHA production.
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